The application of high temperature treatment to food is one of the most common methods for eliminating microbial contamination. In this study, high temperature tolerance of Salmonella Derby（SD）was obtained by high temperature culture and the effect of the heat-stressed SD on its response to environment stresses The survival rate of high temperature domesticated SD was 1.5 time higher in 55 ℃ for 20 min than the control group. The survival rate of high temperature domesticated SD was better in pH 3.0 and pH 2.4 acid challenge. The high temperature tolerant bacteria had enhanced sensitivity to alkali, ethanol and NaClO. The survival of the control group was 33.83%, but the high temperature tolerance SD did not survive in pH 12 for 30 min. On the other hand, The survival rate of thermotolerant SD was 30.05% better than the control group under 125 mmol/L H2O2 stress. The control group was more susceptible to -20 ℃. Temperature stress enhance tolerant SD to acid, H2O2 and -20 ℃ low temperature and sensitivity to alkali, ethanol and NaClO. The combination of environmental stress helps to improve the sterilization effect of thermotolerant SD.