Titration acidity of different buffalo raw milk samples were investigated in both in the same and different season according to GB 4789.18—2010 of “milk and dairy products inspection”. Among the samples, 228 of them were from major buffalo farming community in Yunnan Province, and 655 were from 26 farmers. The alcohol and alkali-doped were test, and the composition of the diet, feeding patterns and parity and lactation days of buffalo were also surveyed. The results showed that the titratable acidity of milk buffers with milk titers ranging from 10 to 13 °T was accounted for 85.7%, and 10.6% of the titratable acidity was more than 14 °T. Only 3.6% in 883 samples was found their acidities were less than 10 °T. Moreover, 83.8% in 228 samples of different origins at the same season were found with the titratable acidity ranging from 10 to 13 °T, and the titratable acidity of 86.4% in 655 samples from same origins at the different season ranged from 10 to 13 °T.