Peptides were prepared form enzymatic hydrolysis of whitmania pigra protein by composite enzyme(papain and flavorzyme) and then made to react with zinc sulfate heptahydrate under certain conditions to produce polypeptide-zinc chelates that qualitative analysis by sodium sulfide method . The structure of polypeptide-zinc chelates was preliminary research by UV, IR, Fluorescence, X diffraction analysis technology to polypeptide and polypeptide-zinc chelates. The results show that the pH of the reaction system,the mass ratio of polypeptide and zinc,the concentration of whitmania pigra polypeptide have a great influence on the reaction of whitmania pigra peptides and zinc sulfate heptahydrate; By comparing analysis the ultraviolet, infrared, fluorescence, X diffraction’s spectras of whitmania pigra polypeptides and polypeptide-zinc chelates, significant changes we found between the spectras, indicating the polypeptide structure produced a significant change after chelation reaction by adding zinc; Finally, according to the polypeptide and polypeptide-zinc chelate’s infrared spectrums and structure characteristic of metal-polypeptide chelates, a possible structure of whitmania pigra peptides-zinc chelates have been inferred.